Biological Sciences
Courses Taught:
- Biology, Anatomy and Physiology
- PhD, 1986, Aligarh Muslim University
- MPhil, 1984, Aligarh Muslim University
- MS, 1980, Aligarh Muslim University
- BS (Hons), Aligarh Muslim University
- O level, 1974, Cambridge University,UK
Research Interests:
- Stem/Progenitor Cell Research: Isolation of progenitor cells from human and rat hippocampuss. Generating adult and fetal stem cells. Analysis of cellular models in Alzheimer's disease and related disorders. Establishment of organotypic hippocampal slices in culture and studying the effect of NMDA receptor antagonists in regulation of cellular phosphorylation-dephosphorylation balance. Studying the effect of growth factors in modulating the neurogenesis in animal and cell models and the effect of molecules in signal transduction. Role of tau phosphorylation by glycogen synthase kinase-3ß in tau-dominated small caliber and effect on subcellular organnels
Fields of interest:
- Microtubule associated protein Tau as a DNA-Chaperone
- Association of proteins with chromosomes in metaphase
- Studies on Fetal and Adult progenitor cells derived from rat and human hippocampus
- Establish organotypic rat and human brain slices in culture and study the effect of progenitor cells on plasticity and neurogenesis
- Cloning, expression and purification of human tau protein: Establish cell culture models of human tau and study regulation of gene expression on cell maturation and plasticity
- Studies on stem cells and cytokine regulation in disease
and health
Publications:
- Haque, N., Rizvi, S.J. and Khan, M.B. Malathion induced alterations in the lipid profile and the rate of lipid peroxidation in rat brain and spinal cord. Toxicology and Pharmacology 61:11-15, 1987.
- Haque, N., Denman, R.B., Merz, G., Grundke-Iqbal, I. and Iqbal, K. Phosphorylation and accumulation of tau without any concomitant increase in tubulin levels in Chinese hamster ovary cells stably transfected with Human tau441. FEBS Lett. 360:132-136, 1995.
- Singh, T.J., Haque, N., Grundke-Iqbal, I. and Iqbal, K. Rapid Alzheimer-like phosphorylation of tau by the synergistic actions of non-proline-dependent protein kinases and GSK-3. FEBS Lett. 358:267-272, 1995.
- Iqbal, K., Alonso del C., A., Gong, C.-X., Haque, N., Khatoon, S., Pei, J.J., Singh, T., Tanaka, T., Wang, J.-Z. and Grundke-Iqbal, I. Role of neurofibrillary degeneration in Alzheimer disease. In: Aging of the Brain and Alzheimer Disease, edited by Z.C. Cheng, P.R. China, 1995.
- Haque, N., Tanaka, T., Iqbal., K., and Grundke-Iqbal I. Regulation of expression, phosphorylation and biological activity of tau during differentiation in SY5Y cells. Brain Res. 838:69-77, 1999.
- Haque, N., Zhong, J., Iqbal, K. and Grundke-Iqbal, I. Overexpression of tau associates with terminal differentiation of neuronal cells in culture. Abstract, 7th International Conference on Alzheimer Disease and Related Disorders, Washington D.C. 2000.
- Hua Q, He, R.Q., Haque, N., Qu, M.H., del Carmen Alonso A, Grundke-Iqbal, I. and Iqbal, K. Microtubule associated protein tau binds to double-stranded but not single-stranded DNA.Cell Mol Life Sci. 2003 Feb;60(2):413-21.
- Wen, G.Y.,Jenkins, E.C., H., Grundke-Iqbal, I., Haque, N., Genovese, M., Chen, J., and Kozlowski, P.B. Tau, ubiquitin, tubulin, and amyloid beta proteins in neuroblastoma metaphase chromosomes. J. Neuropathol Expt. Neurology 2003/
- Iqbal, K.,. Alonso, A. del C, El-Akkad,E., Gong, C-X. ,Haque, N, S. Khatoon, Pei, J.J., Tanimukai, H., Tsujio, Iwang,J.Z..and I. Grundke-Iqbal. Alzheimer neurofibrillary degeneration: therapeutic targets and high-throughput assays. J Mol Neurosci. 2003;20(3):425-9.
- Tatebayashi, Haque, N., Iqbal, K. and I., Grundke-Iqbal. Role of tau phosphorylation by glycogen synthase kinase-3ß in tau-dominated small caliber. J. cell Sci.,2003 (in press).
- Haque, N., Gong, C.-X., Sengupta, A., Iqbal, K. and Grundke-Iqbal, I. Role of phosphatases and kinases on the regulation of tau phosphorylation and its biological activity. 2004 (communicated).
- Liang, Li; Haque, N., Sengupta, A., Grundke-Iqbal, I. and Iqbal, K. Memantine restores the okadaic acid-induced changes in the activities of protein phosphatase-2A and calcium, calmodulin-protein kinase II and hyperphosphorylation of tau in rat hippocampal slices in culture. 2004 (communicated).
- Haque, N., Iqbal, K. and Grundke-Iqbal, I. Regulation of expression and biological activity of microtubule associated proteins in a cell culture model. 2004 (in preparation).
Abstracts:
- Haque, N. and Hasan M. Observation on alterations
in the ATPase level in various regions of the rat brain and
spinal cord following three different doses of dichlorvos. Proceedings,
International Brain Research Organization Symposium on “Neurotoxic
Substances and their impact on Human Health” and 3rd Scientific
Meeting of Indian Academy of Neurosciences, Industrial Toxicology
Research Center, Lucknow, India, 1984.
- Haque, N.,Rizvi, S.J., and Hasan M. Studies
on the effect of an organophosphate Malathion on the discrete
regions of the brain and spinal cord of rat. Proceedings, XXXth
Annual Conference of the Association of Physiologists and Pharmacologists
of India, Goa Medical College, Bambolim, Goa, India, 1984.
- Rizvi, S.J., Haque, N., and Bano, A. Effect
of Malathion on RNA, DNA turnover : Correlation with histopathological
alterations in the CNS of rat. Proceedings, XXXIst Annual Conference
of the Association of Physiologists and Pharmacologists of India,
University of Calcutta, Calcutta, India, 1985.
- Rizvi, S.J., Haque, N. A study of acute neurotic
effects of Dhatura Stramonium.Effect. Proceedings, VIIth Annual
Conference of the Indian Academy of Forensic Medicine, Medical
College, Trivandrum, India, 1985.
- Haque, N. and Khan, M.B. Organophosphorus
Pesticide induced neurochemical changes in CNS of rat. Proceedings.
International Conference on Pesticides: Toxicity, Safety and
Risk Assesment. Industrial Toxicological Research Center, Lucknow,
India, 1985.
- Haque, N. and Khan, M.B. Dichlorvos induced
alterations in acetylcholine content of rat CNS: Interaction
with 2 PAM. Proceedings, Society of Biological Chemists of India,
54th Annual Meeting, G.B. Pant University of Agriculture and
Technology, Pantnagar, India, 1985.
Rizvi, S.J., Haque, N., and Bano, A. Malathion induced biochemical alterations in rat: Correlation with histopathological changes in the CNS and other organs of the body. Proceedings, VIIIth Annual Conference of the Indian Academy of Forensic Medicine, Grant Medical College, Bombay, India, 1986.
- Haque, N. and Ziu, M.M. Studies on the effect
of an organophosphate Dichlorvos on the brain lipid metabolism
and fatty acid profile. Proceedings, XIIth International Meeting
of the International Association of Forensic Sciences, Adelaide,
Australia, 1990.
- Haque, N., Denman, R.B., Grundke-Iqbal, I.,
and Iqbal, K. Overexpression of Human Tau 441 in CHO cells.
Neurobiol. Aging, 15(Supplement I):S (Abstract #68). Fourth
International Conference on Alzheimer’s Disease and Related
Disorders, Minneapolis, Minnesota, USA, 1994.
- Denman, R.B.,Smedman, M., Haque, N.,Kung,
L. and Sung, Y. b-APP mRNA cleavage by symmetric and asymmetric
hammerhead ribozymes. Keystone Symposia on Molecular and Cellular
Biology: Ribozymes, Basic Science and Therapeutic Applications,
Breckenridge, Colorado, USA, 1995.
- Denman, R.B.,Smedman, M., Haque, N. and Kung,
L. Assymetrically distributing base pairing between helices
I and III of trans-acting hammerhead ribozymes targeteted to
b-APP mRNA does not enhance their activity. Keystone Symposia
on Molecular and Cellular Biology: Ribozymes, Basic Science
and Therapeutic Applications, Breckenridge, Colorado, USA, 1995.
- Iqbal, K., Alonso, A. del C., Gong, C-X., Haque, N.,
Khatoon, S. Pei, J. J., Singh, T.J., Tanaka, T., Wang, J.Z.
and Grundke-Iqbal, I. Mechanisms of Alzheimer neurofibrillary
degeneration and pharmacological imolications. Am. Neuropsychology
Meeting, San Juan, Peurto Rico, 1995.
Grundke-Iqbal, I, Tanaka, T., Tung, Y.C., Haque, N., and Iqbal, K. Abnormal hyperphosphorylation of tau: an early and most significant deleterious event in neurofibrillary degeneration. Fifth International Conference on Alzheimer’s Disease and Related Disorders, Osaka, Japan, 1996.
- Haque, N., Tanaka, T., Iqbal, K and Grundke-Iqbal,
I. Differentiation of SY5Y cells: effect on phosphorylation
and biological activity of tau. Fifth International Conference
on Alzheimer’s Disease and Related Disorders, Osaka, Japan,
1996.
- Haque, N., Iqbal, K and Grundke-Iqbal, I.
Regulation of expression and biological activity of microtubule
associated proteins in a cell culture model. Sixth International
Conference on Alzheimer’s Disease and Related Disorders,
Amsterdam, The Netherlands, 1998.
- Haque, N., Zhong, J., Iqbal, K and Grundke-Iqbal,
I. Overexpression of Tau associates with differentiation of
neuronal cells in culture. Seventh International Conference
on Alzheimer’s Disease and Related Disorders, Washington
DC, USA, 2000.
- Grundke-Iqbal, I., Tatebayashi, T., Zhong, J., Haque,
N., and Iqbal, K. Role of protein kinases, protein
phosphatases and extracellular signals in the abnormal hyperphosphorylation
of tau. Eight International Conference on Alzheimer’s
Disease and Related Disorders, Washington DC, USA, 2000.
- Haque, N., Thakkar, P., Iqbal, K and Grundke-Iqbal,
I. Transfection of Neural Progenitor Cells from Fetal Human
Brain with adult human tau induces maturation into neurons.
Presented at the Eight International Conference on Alzheimer’s
Disease and Related Disorders, Stockholm, Sweden, 2002.
- Wen, G.Y.,Jenkins, E.C., H., Grundke-Iqbal, I., Haque,
N., Genovese, M., Chen, J., and Kozlowski, P.B. Tau,
ubiquitin, tubulin, and amyloid beta proteins in neuroblastoma
metaphase chromosomes. Presented at Orlando, Florida in June,
abstract in J. Neuropathol Expt. Neurology 2003
- Haque, N., Gong, C-X, Sengupta, A., Iqbal,
K and Grundke-Iqbal, I. Regulation of microtubule-associated
proteins, protein kinases and protein phosphatases during differentiation
of SY5Y cells. To be presented at the Eight International Conference
on Alzheimer’s Disease and Related Disorders, Philadelphia,
USA, 2004.
- Chohan, M.O., Alonso, A., Haque, N., El-Akkad,
E., Grundke-Iqbal, I.,Grover, A., Iqbal, K. Hyperphosphorylation
induced self-assembly of murine tau: a comparision with human
tau. To be presented at the Eight International Conference on
Alzheimer’s Disease and Related Disorders, Philadelphia,
USA, 2004.
